Selection of Seeds
Just like other grown from seed, to get a quality crop that made the first step is the selection of a good seed seeds. The trick is to soak the beans for 2 nights or 48 hours with water. The characteristics of a good seed and good quality are the seeds sink into the water. while the floating seeds have less good quality. Choose seeds that sank just to be planted for maximum results.
Seeding is done to get a good crop seeds, because later will determine the outcome of the plants having grown in the field or forest for planting the seeds of the sandalwood. What should be done in seeding are: Prepare the black soil has digemburkan, sow the seeds that have been soaked and had sorted above baiak quality seedlings had previously watered evenly. Then sprinkle a little ground black but do not cover the whole bean. For a faster flush percambahan seeds planted every morning and afternoon. The next prepare small polybags filled with black soil. If the seeds are planted has been broken and pulled out a small bud immediately move into a polybag.
Once the seedlings grow as high as 15 cm move the seedlings into the planting spot. The steps are as follows.
• Determine the location field, select the location of water sufficient land, meaning that no dry land as dry land sandalwood tree can not grow well.
• Clean the area of weeds or wild plants, cut trees blocking the sunlight, then gemburkan growing media beforehand.
• Dig a hole the size 40x40cm with a depth of 50cm. planting hole spacing of at least 2.5 m.
• Insert manure / organic fertilizer into the hole with a thickness of 10cm and insert the sandalwood tree seedlings that had been prepared, cover the hole with soil excavation back and leaving 5cm (to hold water so as not to widen out).
• Then plug the marker as a marker.
Planting time should afternoons.
Watering is done 2 times a day ie morning and afternoon. Sandalwood does not require much water watering pretty much as 800 cc or 3-4 glasses of water each stem of the plant.
Weeding is cleaning activities of sandalwood plants so that the plants nuisance weeds sandalwood unrivaled in the absorption of nutrients. Weeding is done 2 times a year or adjusted to the state of weed growth. Weeding is usually done early in the day with grass or reeds, which interferes with plant growth sandalwood.
Fertilization is done 2 times a day at the beginning of the rainy season and the end of the rainy season. Basic fertilizer when planting sandalwood is a mixture of manure and urea (500 g of manure and 5 g urea / hole). Step in giving the fertilizer is dig a hole as deep as 35 cm, create a circular hole put around the hole excavated soil and fertilizer inputs which have been mixed earlier into the hole, piled back in the dug soil earlier, and then condense the land.
Pruning is done by reducing the branches that grow excessive, aims to improve the quality of a great tree trunks and the host plant does not interfere with plant growth sandalwood, as well as trigger the growth of new shoots. The trick prune branches that are old plants with menggunak machete or chainsaw.
Propagation Sandalwood (Santalum album Linn.) In culture in vitro by administration of plant growth regulator cytokinin (BAP and Kinetin)
Sandalwood (Santalum album Linn.) Is a typical timber forest products from East Nusa Tenggara (NTT) and East Timor (East Timor). This plant has high economic value, because it can produce essential oils with aroma spesif1k, as a basic ingredient of perfume, soap and incense and have properties as a reliever seizures, nausea and fever. Cendana existence now is a rare plant, it is recorded in the 1994 IUCN Red List Threatened Species. Therefore, immediate action is taken cultivation, among others through in-vitro culture by providing growth regulator (PGR) cytokinin is BAP (6-Benzylaminopurine), Kinetin (6¬furfurylaminopurine) and combinations thereof. Cendana order to get the optimal reproduction, this research needs to be done. This study aims to determine the effectiveness of plant growth regulator cytokinin group that BAP, Kinetin, or a combination thereof at Cendana propagation.
Research activities take place in the Laboratory of Tissue Culture Unit Tumouhan Conservation Department of Forest Resources Conservation and Ecotourism Faculty of Forestry IPB, for 5 months from May to September 2005. The experimental design used in this research is completely randomized design (CRD) with 1 factorial is giving PGR form of BAP, Kinetin and combinations thereof, are given on Murashige and Skoog (MS) consists of 10 treatments with each treatment 10 replicates. Broadcaster Al (BAP 0 mg / I: Kinetin 0 mg / I), A2 (BAP 0.5 mg / I: Kinetin 0 mg / I), A3 (BAP I mg / I: Kinetin 0 mg / I), A4 (BAP 1.5 mg / I: Kinetin 0 mg / I), US (BAP 2.0 mg / I: Kinetin 0 mg / I), A6 (BAP 0 mg / I: Kinetin 0.2 mg / I), A7 (BAP 0.5 mg / I: Kinetin 0.2 mg / I), A8 (BAP 1.0 mg / I: Kinetin 0.2 mg / I), A9 (BAP 1.5 mg / I: Kinetin 0.2 mg / I), and AIO (BAP 2.0 mg / I: Kinetin 0.2 mg / I ). Variables were observed and measured are visual observations, the number of shoots, the number of books, height and number of leaves.
Based on visual observations of contamination, but fairly low at 17%. Eksplan shoots are used to show symptoms of browning, especially on the dipotorig. There is a callus growth, but does not dominate in every treatment, only found in a few explants on A9 treatment (BAP 1.5 mg / I: Kinetin 0.2 mg / I). Leaf loss occurs up to a percentage of 15.61%, then action Glutamine subculture with the addition of 100 mg / I in the media. The average percentage of leaf loss has decreased by 6.70%.
Results of analysis of variance showed that administration of cytokines that dossier PGR provides highly significant effect, while Kinetin and the combination does not give effect to the number of shoots. Average value
the largest increase in the number of shoots contained in A9 is the media treatment of MS with the addition BAP1.5 mg / I and Kinetin 0.2 mg / I with a rate of 1:40, while the lowest average value contained in the treatment of Al (BAP 0 mg / I: Kinetin 0 mg / 1) and A3 (BAP 1 mg / 1: Kinetin 0 mg / 1) with a rate of 0:00.
Based on the analysis of variance showed that the dossier PGR provides highly significant effect, while Kinetin and the combination does not give effect to increase the number of books. The average value of the largest in the number of books are on the A9 treatment (BAP 1.5 mg / I: Kinetin 0.2 mg / l) is 4:40, while on treatment A6 (BAP 0 mg / I: Kinetin 0.2 mg / l) give effect to the mean Lowest book pertambahanjumlah average of 1:50.
Results of analysis of variance showed that the dossier PGR provides highly significant effect, Kinetin effect was not significantly different and significantly different combination effect against high eksp1an. Can be seen the average value of the largest high gain A9 that is contained in the treatment of MS medium with the addition of a combination of BAP 1.5 mg / 1 and Kinetin 0.2 mg / I shows the number increment at 1:40 em high, while the average value of the high gain lowest for the treatment A6 with the provision of Kinetin 0.2 mg / 1 shows the number accretion 0:48 em.
Based on the analysis of variance showed that the PGR BAP, Kinetin and combinations give effect to the accretion jum1ah-1 leaf stage. The average gain of the biggest high is contained in the treatment A3 Media MS with the addition of BAP 1 mg / I with the number added at 13:20 strands, while the lowest average value contained in the treatment A6 (BAP 0 mg / I: Kinetin 0.2 mg / I) with a rate of 2.80 strands.
Based on the analysis of variance granting Kinetin PGR provides highly significant effect, while the BAP and the combination does not affect the accretion jum1ah leaves on the 2nd stage of observation. On average in the number of the largest leaf 'contained in the A4 is the media treatment of MS with the addition of BAP 1.5 mg / I with a value of 4:30 he1ai, while the lowest was in the number of leaves on the A6 treatment (BAP o mg / I: Kinetin 0.2 mg / 1) with ni1ai 0:50 strands.
General Seeara A9 treatment by administering a combination of PGR namely BAP 1.5 mg / I and Kinetin 0.2 mg / I shows the average value of the variable gain jum1ah best buds, the number of books and high eksp1an Cendana. The average gain jum1ah leaf stage 1st highest ni1ai contained in A3 treatment by administering BAP concentration of 1 mg / 1, while the increase jum1ah leaf stage 2nd highest value contained in A4 treatment with BAP concentration of 1.5 mg / I. Glutamine addition of 100 mg / 1 in the media with the addition of BAP and Kinetin managed to reduce the loss of leaves.